Waimea Valley on the North Shore of Oahu, site of the Keck Ecosystem Microbiome Observatory

Waimea Valley on the North Shore of Oahu, site of the Keck Ecosystem Microbiome Observatory

Postdoctoral Fellow: Microbial Ecology and Evolutionary Genomics

Description: A NIH-funded postdoctoral position with full benefits is available in Dr. Joanne Yew’s laboratory for a highly motivated candidate interested in studying the environmental and genetic functions that govern microbiome diversity in endemic Hawaiian Drosophila species. The Yew lab is part of the newly established NIH-funded Integrative Center for Environmental Microbiomes and Human Health in the Pacific Biosciences Research Center at the University of Hawaiʻi at Mānoa, led by Drs. Margaret McFall-Ngai and Edward Ruby (http://www.pbrc.hawaii.edu/).

The project will involve 1) field collection and lab culturing of Hawaiian drosophilids; 2) generation of metagenomic datasets; 3) small molecule and lipid analysis by mass spectrometry; 4) dietary manipulation and physiological/ behavioral assays; 5) generation of axenic animals.

Desired qualifications: The ideal candidate will have a Ph.D. in microbial ecology, evolutionary genomics, biochemistry, or chemical ecology. Preferred qualifications include a track record of innovative and integrative research with experience in standard molecular biology and microbiology techniques, bioinformatic analysis of metagenomic and transcriptomic datasets, physiological and behavioral analysis, and a strong interest in field work. The approximate length will be up to 36 months with the potential for extension.

Contact: Interested  candidates  should  send  an  application  packet  by  email  to  Dr.  Joanne  Yew (jyew@hawaii.edu) containing:

  • Cover letter indicating current and future research interests

  • Expected start date

  • Curriculum vitae

  • Names and contact information for 3 references

Application deadline: 15 November 2018


Postdoctoral Fellow/ Staff Scientist: Environmental Microbiomes

Description: The newly established NIH-funded Integrative Center for Environmental Microbiomes and Human Health in the Pacific Biosciences Research Center at the University of Hawaiʻi at Mānoa, led by Drs. Margaret McFall-Ngai and Edward Ruby, is seeking a productive and highly motivated staff scientist to oversee the daily operations of the Microbial Genomics and Analytical Laboratory (MGAL) and Center for Invertebrate Science Training and Research (C-InSTaR) core facilities. The primary mission of MGAL is to provide services and infrastructure to address the needs of Hawai‘i microbiome researchers. Services include processing of environmental samples, library preparation for genomic and transcriptomic analysis, culturing and storage of microbes, quantitative small molecule and lipid analysis, and maintenance of the Hawaiʻi Microbe Catalog (HIMC), a collection of microbial isolates found in the Pacific region. C-InSTaR will house invertebrate experimental organisms including mosquitoes, Drosophila, and gastropods.

Desired qualifications: The ideal candidate will have a Ph.D. in microbiology, molecular biology or related fields, and experience with microbial culturing, transcriptomics and genomics, biochemical purification, and bioinformatics. Applicants should have a desire to work in a multi-disciplinary team of investigators, excellent scientific writing and oral communication skills, and a history of effective collaboration. Experience with insect husbandry is desired but not required.

Responsibilities:

  • Establish infrastructure and develop a workflow for MGAL services including nucleic acid extraction from a wide range of samples (including soil, water, plants, animals, and air filters), library preparation, and quantitative PCR

  • Develop and implement protocols for mass spectrometry-based quantitative lipid analysis of plant and animal tissue to support facility user needs

  • Supervise and train MGAL research staff (including one full-time technician), student assistants, and core facility users on lab safety, conduct, standards, and technical practices

  • Pursue an independent research project complementary to the research aims of Center that utilize Drosophila or mosquitoes as a model. Potential projects include but are not limited to the functional roles of the microbiome on host physiology and disease transmission. The project will be mentored by Center PIs Dr. Matthew Medeiros and Joanne Yew.

  • Curate and organize the HIMC microbial strain collection and database

  • Liaise with university administration and state and national agencies on matters related to lab safety certification and import and export permits

  • Maintain animal cultures and equipment in C-InSTaR

  • The successful candidate is expected to devote 60% of their time to MGAL and C-InSTaR activities and 40% of their time to the independent research project. Funding is available for up up to 5 years, with the potential for extension.

Contact: Interested applicants should submit a cover letter, CV, and contact information for three references to Drs. Matthew Medeiros (mcmedeir@hawaii.edu) and Joanne Yew (jyew@hawaii.edu).

Application deadline: 15 November 2018